Abstract:

 This experiment was conducted to detect existence of GnRHR in caudal mesenteric ganglion (CMG) of female goat，and discuss the possible functional role of GnRHR in regulating reproductive activity through endocrine and autonomic nerve pattern.Immunohistochemical SP method was used to observe the distribution characteristics of GnRHR in CMG.The results showed that all the neurons in CMG were responded positively to GnRHR.The membrane and whole cytoplasm of neurons were strong positive stained with brown and the neurite was middle positive stained with yellow to GnRHR.Weak GnRHR immunoreactivity products distributed on passing nerve fibers and satellite cells.Image analysis documented a significant difference of GnRHR expression between neurons and non-neurons (P＜0.01).The results suggest that CMG’s neurons are the major targets of GnRH，which implies GnRH may regulate the function of reproductive organs through the GnRHR distributed in CMG.Therefore GnRHR may be a “transfer station” to coordinate reproductive activity through endocrine and autonomic nerve pattern.